Difference between revisions of "Part:BBa K411101:Design"

 
(Source)
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K411101 short</partinfo>
 
<partinfo>BBa_K411101 short</partinfo>
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===Source===
 
===Source===
  
PCR of GFP(from biobrick) and eIF4A(from C.Proud)
+
PCR fusion of GFP (BBa_Kxxxxxx) and eIF4A (BBa_K411100). More details of the fusion can be found on [http://2010.igem.org/Team:NYMU-Taipei/ our wiki page].
  
 
===References===
 
===References===

Revision as of 17:01, 8 October 2010

GFP_split_A + Linker + eIF4A_split_A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 741
    Illegal BglII site found at 1037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1139


Design Notes

the split site must be fixed!


Source

PCR fusion of GFP (BBa_Kxxxxxx) and eIF4A (BBa_K411100). More details of the fusion can be found on [http://2010.igem.org/Team:NYMU-Taipei/ our wiki page].

References