Difference between revisions of "Part:BBa K322211:Design"

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===Design Notes===
 
===Design Notes===
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Sequence date: the mutation was initially introduced into our construct K216015 and the sequence across the mutation was obtained on 12 Aug 2010 (clone 4). The coding sequence was then amplified by PCR and cloned in pSB1C3 by Will and Lu.
 
  
 +
This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, which were presumably present in BBa_I712019 to make a proper eukaryotic context for the start codon, were removed for improved expression in ''E. coli''.
  
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Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.
  
 
===Source===
 
===Source===

Revision as of 18:54, 7 October 2010

Red emitting firefly luciferase mutant (356K)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, which were presumably present in BBa_I712019 to make a proper eukaryotic context for the start codon, were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.

Source

Photinus pyralis, from BBa_I712019

References

Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007).