Difference between revisions of "Part:BBa K322210"

Revision as of 18:39, 7 October 2010

Chloramphenicol acetyltransferase (cat)

cat is an antibiotic resistance gene, used in the first step of BRIDGE which requires the deletion of existing DNA (probably a non-coding piece or a non-essential gene) to select for cells which have taken up the construct by growing them on the relevant antibiotic.

Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions.

Notes: has complete suffix but prefix lacks NotI site. Still completely compatible with RFC10.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 cat is an antibiotic resistance gene, used in the first step of BRIDGE which requires the deletion of existing DNA (probably a non-coding piece or a non-essential gene) to select for cells which have taken up the construct by growing them on the relevant antibiotic.
-In the BRIDGE, with this system, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with new genes.
+Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions.
+Notes: has complete suffix but prefix lacks NotI site. Still completely compatible with RFC10.
-Notes: has complete suffix but prefix lacks NotI site.
-Features: coding sequence runs from base 294 to 944, rbs 280 to 285.