Difference between revisions of "Part:BBa K330000:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | FLAG-tag is a polypeptide protein tag which is commonly used in molecular biology, particularly recombinant DNA technology. It can be added to the N-terminal, C-terminal or within a protein coding sequence. FLAG-tag is used in affinity chromatography to separate or detect recombinant, overexpressed protein from wild-type protein; it can also be used in cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis. | ||
===Source=== | ===Source=== | ||
Revision as of 14:11, 7 October 2010
Protein Tag: FLAG-tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
FLAG-tag is a polypeptide protein tag which is commonly used in molecular biology, particularly recombinant DNA technology. It can be added to the N-terminal, C-terminal or within a protein coding sequence. FLAG-tag is used in affinity chromatography to separate or detect recombinant, overexpressed protein from wild-type protein; it can also be used in cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis.
Source
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