Difference between revisions of "Part:BBa K389014:Design"
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<partinfo>BBa_K389014 short</partinfo> | <partinfo>BBa_K389014 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | medium strong constitutive | + | * The ''virA'' gene is under the control of a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein) |
| − | + | * double terminator (forward) to keep expression of kanamycin resistance by the constitutive promoter low | |
| − | + | ** double terminator <partinfo>B0017</partinfo> instead of <partinfo>B0015</partinfo> because of problems mentioned with <partinfo>B0012</partinfo> | |
| + | * only for testing the screening principle of mutated ''virA'' screening | ||
===Source=== | ===Source=== | ||
Revision as of 12:51, 7 October 2010
VirA/G screening system test
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768
Design Notes
- The virA gene is under the control of a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)
- double terminator (forward) to keep expression of kanamycin resistance by the constitutive promoter low
- only for testing the screening principle of mutated virA screening
Source
A. tumefaciens C58 TI-plasmid, parts.igem, Mr. Gene