Difference between revisions of "Part:BBa K389011:Design"
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===Design Notes=== | ===Design Notes=== | ||
* medium strong constitutive promoter, | * medium strong constitutive promoter, | ||
| − | * double terminator (forward) to keep | + | * double terminator (forward) to keep expression of kanamycin resistance by the constitutive promoter low |
** double terminator <partinfo>B0017</partinfo> instead of <partinfo>B0015</partinfo> because of problems mentioned with <partinfo>B0012</partinfo> | ** double terminator <partinfo>B0017</partinfo> instead of <partinfo>B0015</partinfo> because of problems mentioned with <partinfo>B0012</partinfo> | ||
* kanamycin resistance for screening with plates | * kanamycin resistance for screening with plates | ||
Revision as of 12:46, 7 October 2010
VirA screening device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- medium strong constitutive promoter,
- double terminator (forward) to keep expression of kanamycin resistance by the constitutive promoter low
- kanamycin resistance for screening with plates
Source
- virG gene synthesized by Mr. Gene (BBa_K389002)
- vir promoter from A. tumefaciens C58 (BBa_K389003)
- kanR gene made from BioBrick BBa_P1003
- constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)