Difference between revisions of "Part:BBa K389011:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K389011 short</partinfo>
 
<partinfo>BBa_K389011 short</partinfo>
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===Design Notes===
 
===Design Notes===
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medium strong constitutive promotor, terminator to keep basal expression of kanamycin resistance low
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* medium strong constitutive promoter,  
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+
* double terminator (forward) to keep basal expression of kanamycin resistance low
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** double terminator <partinfo>B0017</partinfo> instead of <partinfo>B0015</partinfo> because of problems mentioned with <partinfo>B0012</partinfo>
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* kanamycin resistance for screening with plates
  
 
===Source===
 
===Source===

Revision as of 12:30, 7 October 2010

VirA screening device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • medium strong constitutive promoter,
  • double terminator (forward) to keep basal expression of kanamycin resistance low
  • kanamycin resistance for screening with plates

Source

synthetic virG gene, vir promotor from A. tumefaciens C58, parts.igem

References