Difference between revisions of "Part:BBa K411100:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Mutated | + | Mutated two PstI cutting sites at positions 223 and 391 from ctgcag to ctccag. <!-- See our wiki for the details of the PCR primers used to do the point mutation --> |
| − | + | ||
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===Source=== | ===Source=== | ||
Latest revision as of 07:13, 7 October 2010
Eukaryote Initiation Factor 4A (eIF4A) from Mus Musculus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 240
Illegal BglII site found at 536 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 972
Illegal BsaI.rc site found at 638
Design Notes
Mutated two PstI cutting sites at positions 223 and 391 from ctgcag to ctccag.
Source
[http://www.ncbi.nlm.nih.gov/nuccore/NM_144958 eIF4A] from Mus Musculus