Difference between revisions of "Part:BBa K389001:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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An illegal ''PstI'' restriction site was removed, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.  
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An illegal ''PstI'' restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.
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+
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===Source===
 
===Source===

Latest revision as of 13:42, 4 October 2010

VirA receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 586
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1707


Design Notes

An illegal PstI restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.

Source

Agrobacterium tumefaciens C58, TI-plasmid

References