Difference between revisions of "Part:BBa K389001:Design"

 
(Design Notes)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K389001 short</partinfo>
 
<partinfo>BBa_K389001 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
Illegal ''PstI'' restriction site removed
+
An illegal ''PstI'' restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 13:42, 4 October 2010

VirA receptor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Unknown


Design Notes

An illegal PstI restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.

Source

Agrobacterium tumefaciens C58, TI-plasmid

References