Difference between revisions of "Part:BBa K389001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | An illegal ''PstI'' restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added. | |
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===Source=== | ===Source=== | ||
Latest revision as of 13:42, 4 October 2010
VirA receptor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 586
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1571
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1707
Design Notes
An illegal PstI restriction site was removed by site-directed mutagenesis, the startcodon was changed to ATG and the stop codon to TAA. A second TAA stop codon was added.
Source
Agrobacterium tumefaciens C58, TI-plasmid