Difference between revisions of "Part:BBa K302017"
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Coding sequences and RBS' for spaIFEG cluster.
 
Coding sequences and RBS' for spaIFEG cluster.
 
SpaI — Membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.  
 
SpaI — Membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores.  
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SpaFEG — three proteins from B. Subtilis which form a membrane transporter that removes subtilin from the membrane, preventing it from forming pores and killing the cells.
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Revision as of 11:12, 28 September 2010

spaIFEG cluster

Coding sequences and RBS' for spaIFEG cluster. SpaI — Membrane bound protein that binds subtilin and prevents it from penetrating the cell membrane to form pores. SpaFEG — three proteins from B. Subtilis which form a membrane transporter that removes subtilin from the membrane, preventing it from forming pores and killing the cells.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
    Illegal BglII site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1035
    Illegal SapI site found at 1315
    Illegal SapI.rc site found at 2038