Difference between revisions of "Part:BBa K318030"
Line 10: Line 10:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K318030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K318030 SequenceAndFeatures</partinfo>
  

Revision as of 20:28, 27 September 2010

lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC

This composite part is a "lock" designed to prevent expression of a downstream gene unless a specific sequence of recombination events occurs to the part. Specifically, successive application of the Hin and Cre recombinases, respectively, will "unlock" the part leading to constitutive expression of the downstream gene(s). It was refered to as the "Lock Cassette" or "Lock Construct" by the Wisconsin-Madison 2010 iGEM team.

It can be used in conjunction with the Key Cassette after miscellaneous (junk) DNA is added to either the HindIII or the SapI (5' ATC 3' overhang) sites that are located within the part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 43
    Illegal NheI site found at 66
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 162