Difference between revisions of "Part:BBa K318030"
(New page: __NOTOC__ <partinfo>BBa_K318030 short</partinfo> This composite part is a "lock" designed to prevent expression of a downstream gene unless a specific sequence of recombination events occ...) |
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This composite part is a "lock" designed to prevent expression of a downstream gene unless a specific sequence of recombination events occurs to the part. Specifically, successive application of the Hin and Cre recombinases, respectively, will "unlock" the part leading to constitutive expression of the downstream gene(s). It was refered to as the "Lock Cassette" or "Lock Construct" by the Wisconsin-Madison 2010 iGEM team. | This composite part is a "lock" designed to prevent expression of a downstream gene unless a specific sequence of recombination events occurs to the part. Specifically, successive application of the Hin and Cre recombinases, respectively, will "unlock" the part leading to constitutive expression of the downstream gene(s). It was refered to as the "Lock Cassette" or "Lock Construct" by the Wisconsin-Madison 2010 iGEM team. | ||
| − | It can be used in conjunction with the [https://parts.igem.org/ | + | It can be used in conjunction with the [https://parts.igem.org/Part:BBa_K318000 Key Cassette] after miscellaneous (junk) DNA is added to either the HindIII or the SapI (5' ATC 3' overhang) sites that are located within the part. |
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Revision as of 20:13, 27 September 2010
lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC
This composite part is a "lock" designed to prevent expression of a downstream gene unless a specific sequence of recombination events occurs to the part. Specifically, successive application of the Hin and Cre recombinases, respectively, will "unlock" the part leading to constitutive expression of the downstream gene(s). It was refered to as the "Lock Cassette" or "Lock Construct" by the Wisconsin-Madison 2010 iGEM team.
It can be used in conjunction with the Key Cassette after miscellaneous (junk) DNA is added to either the HindIII or the SapI (5' ATC 3' overhang) sites that are located within the part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 43
Illegal NheI site found at 66 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 162