Difference between revisions of "Part:BBa K385005:Design"
I.stansfield (Talk | contribs) (→Design Notes) |
I.stansfield (Talk | contribs) (→Design Notes) |
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It was flanked on the 5' side by; | It was flanked on the 5' side by; | ||
| − | 5' | + | 5' AGATTATCTACTTAA 3' |
and on the 3' side by; | and on the 3' side by; | ||
5' CTTAAGAACACAAAATTCGAGACAT G 3' (note this is 'sense' strand sequence) | 5' CTTAAGAACACAAAATTCGAGACAT G 3' (note this is 'sense' strand sequence) | ||
Latest revision as of 12:34, 27 September 2010
B-box sequence encoding a regulatory mRNA stem loop
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence forms part of a 5' untranslated leader, and so must be free from all AUG sequences. The stem sequence itself was; GGGCCCTGAAGAAGGGCC
It was flanked on the 5' side by; 5' AGATTATCTACTTAA 3' and on the 3' side by; 5' CTTAAGAACACAAAATTCGAGACAT G 3' (note this is 'sense' strand sequence)
Source
Phage lambda genome