Difference between revisions of "Part:BBa K385002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We omitted the stop codon so this part could be used in a protein fusion construct, with the MS2 protein forming the N-terminal domain | + | We omitted the stop codon so this part could be used in a protein fusion construct, with the MS2 protein forming the N-terminal domain. A glycine rich spacer peptide was inserted at the 3' end of the sequence, to allow the N-peptide to be separated from any downstream ORF by a flexible linker. (Linker sequence GGT GAC GGT GCT GGT TTA ATT AAC) |
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===Source=== | ===Source=== | ||
Latest revision as of 11:38, 27 September 2010
Phage MS2 coat protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 331
Design Notes
We omitted the stop codon so this part could be used in a protein fusion construct, with the MS2 protein forming the N-terminal domain. A glycine rich spacer peptide was inserted at the 3' end of the sequence, to allow the N-peptide to be separated from any downstream ORF by a flexible linker. (Linker sequence GGT GAC GGT GCT GGT TTA ATT AAC)
Source
Phage MS2 genome (see NCBI sequence http://www.ncbi.nlm.nih.gov/nuccore/V00642.1)