Difference between revisions of "Part:BBa K371000:Design"

 
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<partinfo>BBa_K371000 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The sequence results of pduAB, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.  
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The sequence results of pduJK, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.
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===Source===
 
===Source===

Revision as of 07:29, 27 August 2010

pduJK(Propanediol utilization gene J+K)[RBS+pduJ+RBS+pduK]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 566
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence results of pduJK, which we got by PCR from Citrobacter Freundii(NBRC NO.12681), are different from the sequence we found on the papar. We suspect that the strain we used is not same with the papar.

Source

pduAb come from Citrobacter Freundii genenom(NBRC NO.12681)

References