Difference between revisions of "Part:BBa I744102"
| Line 4: | Line 4: | ||
Tc -> EYFP | Tc -> EYFP | ||
| − | This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. | + | This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. A transfer function is available in the experience section. |
| − | For more information go to: [http:// | + | For more information go to: [http://2007.igem.org/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs] |
[[image:Tet_sensor.jpg]] | [[image:Tet_sensor.jpg]] | ||
Latest revision as of 02:08, 24 August 2010
Tetracycline sensor with medium-strength promoter
Tc -> EYFP
This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. A transfer function is available in the experience section.
For more information go to: [http://2007.igem.org/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]