Difference between revisions of "Part:BBa I744102"

Latest revision as of 02:08, 24 August 2010

Tetracycline sensor with medium-strength promoter

Tc -> EYFP

This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. A transfer function is available in the experience section.

For more information go to: [http://2007.igem.org/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_I744102 short</partinfo>
-This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement.
+Tc -> EYFP
+This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. A transfer function is available in the experience section.
+For more information go to: [http://2007.igem.org/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs]
+[[image:Tet_sensor.jpg]]
 <!-- Add more about the biology of this part here