Difference between revisions of "Part:BBa J70627:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J70627 short</partinfo> | <partinfo>BBa_J70627 short</partinfo> | ||
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Forward Primer: | Forward Primer: | ||
| − | GTTTCTT C GAATTC GCGGCCGC T ACTAGT | + | GTTTCTT C GAATTC GCGGCCGC T ACTAGT ACGACAAATCCTGGTGTATCCGCTTG |
Reverse Primer: | Reverse Primer: | ||
GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTGAAGCTGCCACAAGGCAGGAAC | GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTGAAGCTGCCACAAGGCAGGAAC | ||
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===Source=== | ===Source=== | ||
Latest revision as of 16:16, 13 August 2010
RFC12 Internal Chitin Binding Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Both sequences in 5' -> 3' direction.
Forward Primer:
GTTTCTT C GAATTC GCGGCCGC T ACTAGT ACGACAAATCCTGGTGTATCCGCTTG
Reverse Primer:
GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTGAAGCTGCCACAAGGCAGGAAC
Source
NEB plasmid: [http://www.neb.com/nebecomm/products/productN6702.asp pTYB2]