Difference between revisions of "Part:BBa K389011"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K389011 short</partinfo> | <partinfo>BBa_K389011 short</partinfo> | ||
This part contains: | This part contains: | ||
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- a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' | - a mutated ''virG'' response regulator from the virA/G receptor system from ''Agrobacterium tumefaciens'' which works in ''Escherichia coli'' without an ''rpoA'' gene from ''A. tumefaciens'' | ||
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- a kanamycin resistance under the control of a ''vir'' promotor | - a kanamycin resistance under the control of a ''vir'' promotor | ||
Revision as of 18:23, 10 August 2010
VirA screening device
This part contains:
- a mutated virG response regulator from the virA/G receptor system from Agrobacterium tumefaciens which works in Escherichia coli without an rpoA gene from A. tumefaciens
- a kanamycin resistance under the control of a vir promotor
This device is used to screen virA receptor mutants. The virA receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more virG is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]