Difference between revisions of "Part:BBa K389011"

Revision as of 18:17, 10 August 2010

VirA screening device

This part contains: - a mutated virG response regulator from the virA/G receptor system from Agrobacterium tumefaciens which works in Escherichia coli without an rpoA gene from A. tumefaciens - a kanamycin resistance under the control of a vir promotor

This device is used to screen virA receptor mutants. The virA receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more virG is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.

Sequence and Features