Difference between revisions of "Part:BBa J70624"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70624 short</partinfo>
 
<partinfo>BBa_J70624 short</partinfo>
  
−
This is a RFC12 compatible FLAG tag meant for N-terminal fusions.  See the RFC10 equivalent, <partinfo>T2004</partinfo> for more information.
+
This is a RFC12 compatible version of <partinfo>BBa_T2004</partinfo>.  It is a FLAG tag head domain meant for n-terminal fusions.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The FLAG tag is a common epitope purification method.  Unlike c-myc tagging, flag tagging yields high enough yields and purity to be used for purification as well as detection, but the same problems with a non-regenerative matrix and low pH elution still apply.
 +
 +
The tag can be cleaved off by enterokinase after the c-terminal lysine.
 +
 +
Common tag information:
 +
<ul>
 +
<li>Protein Sequence: DYKDDDDK </li>
 +
<li>Matrix: Monoclonal Antibody</li>
 +
<li>Yield/Purity: Fairly low yields</li>
 +
<li># Steps: One step</li>
 +
<li>Fusion Location: N-terminus</li>
 +
<li>Affects Function?: Tag will likely not, but the low pH elution may</li>
 +
</ul>
  
 
<!-- -->
 
<!-- -->

Revision as of 15:58, 9 August 2010

RFC12 FLAG Tag Head Domain

This is a RFC12 compatible version of BBa_T2004. It is a FLAG tag head domain meant for n-terminal fusions.

Usage and Biology

The FLAG tag is a common epitope purification method. Unlike c-myc tagging, flag tagging yields high enough yields and purity to be used for purification as well as detection, but the same problems with a non-regenerative matrix and low pH elution still apply.

The tag can be cleaved off by enterokinase after the c-terminal lysine.

Common tag information:

  • Protein Sequence: DYKDDDDK
  • Matrix: Monoclonal Antibody
  • Yield/Purity: Fairly low yields
  • # Steps: One step
  • Fusion Location: N-terminus
  • Affects Function?: Tag will likely not, but the low pH elution may

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]