Difference between revisions of "Part:BBa J70621"
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This is a RFC12 compatible version of <partinfo>BBa_T2000</partinfo>.  It is a 7x his tag tail domain meant for C-terminal fusions.  His tags are meant to help purify a target protein.
 
This is a RFC12 compatible version of <partinfo>BBa_T2000</partinfo>.  It is a 7x his tag tail domain meant for C-terminal fusions.  His tags are meant to help purify a target protein.
  
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===Usage and Biology===
 
===Usage and Biology===
 +
Polyhistidine tags are a very common purification tag used to separate or identify a target protein.
 +
 +
Common tag information:
 +
<ul>
 +
<li>Matrix: Divalent Metal (Ni<sup>2+</sup> is most common)</li>
 +
<li>Yield/Purity: 20%-80% pure, but depending on the methodology can be higher</li>
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<li># Steps: One step</li>
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<li>Fusion Location: C-terminus (N-terminus is <partinfo>J70609</partinfo>)</li>
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<li>Affects Function?: Possible, but rare</li>
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</ul>
  
 
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Revision as of 15:06, 9 August 2010

RFC12 7x His Tag Tail Domain

This is a RFC12 compatible version of BBa_T2000. It is a 7x his tag tail domain meant for C-terminal fusions. His tags are meant to help purify a target protein.

Usage and Biology

Polyhistidine tags are a very common purification tag used to separate or identify a target protein.

Common tag information:

  • Matrix: Divalent Metal (Ni2+ is most common)
  • Yield/Purity: 20%-80% pure, but depending on the methodology can be higher
  • # Steps: One step
  • Fusion Location: C-terminus (N-terminus is BBa_J70609)
  • Affects Function?: Possible, but rare

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]