Difference between revisions of "Part:BBa J70612:Design"

 
(Design Notes)
 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70612 short</partinfo>
 
<partinfo>BBa_J70612 short</partinfo>
Line 9: Line 8:
 
PCR amplified off of <partinfo>BBa_J04450</partinfo> with primers:
 
PCR amplified off of <partinfo>BBa_J04450</partinfo> with primers:
  
GTTTCTT C GAATTC GCGGCCGC T ACTAGT CCAGGCATCAAATAAAACGAAAGGCTC, Term-F
+
GTTTCTT C GAATTC GCGGCCGC T ACTAGT CCAGGCATCAAATAAAACGAAAGGCTC, Term-F
 
+
GTTTCTT C CTGCAG CGGCCGC T GCTAGC TATAAACGCAGAAAGGCCCACCCG, Term-R
+
 
+
  
 +
GTTTCTT C CTGCAG CGGCCGC T GCTAGC TATAAACGCAGAAAGGCCCACCCG, Term-R
  
 
===Source===
 
===Source===

Latest revision as of 22:21, 20 July 2010

RFC12 Double Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR amplified off of BBa_J04450 with primers:

GTTTCTT C GAATTC GCGGCCGC T ACTAGT CCAGGCATCAAATAAAACGAAAGGCTC, Term-F
GTTTCTT C CTGCAG CGGCCGC T GCTAGC TATAAACGCAGAAAGGCCCACCCG, Term-R

Source

BBa_B0015

References