Difference between revisions of "Part:BBa K343000"
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<partinfo>BBa_K343000 short</partinfo> | <partinfo>BBa_K343000 short</partinfo> | ||
| − | The master regulator operon for flagellum synthesis. Produces transcriptional factors for Class II flagellar regulon genes. | + | The master regulator operon for flagellum synthesis. Produces transcriptional factors for Class II flagellar regulon genes. To make the part conform to the iGEM assembly standards we introduced a silent mutation, in an unintended restriction site, at BP 822 from t to c, so that Pst1 would not cleave the DNA there. |
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Revision as of 11:39, 3 July 2010
Mutated flagella master regulator (FlhDC mut).
The master regulator operon for flagellum synthesis. Produces transcriptional factors for Class II flagellar regulon genes. To make the part conform to the iGEM assembly standards we introduced a silent mutation, in an unintended restriction site, at BP 822 from t to c, so that Pst1 would not cleave the DNA there.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 609
- 1000COMPATIBLE WITH RFC[1000]