Difference between revisions of "Part:BBa P1014"
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Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155 | Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155 | ||
| + | Conveys resistance to genamicin at up to 12 ug/ml. It conveys low levels of resistance to kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 4 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance. | ||
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| + | Gentamicin is an important antibiotic for use in human disease. Its use should be carefully considered and alternatives sought when used in a context promoting easy horizontal transfer of the cassette to potential human pathogens, for example in mobilizable plasmids or in transposable elements (insertions sequences). | ||
Latest revision as of 16:35, 2 July 2010
gentamicin resistance cassette aph(2)
Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155
Conveys resistance to genamicin at up to 12 ug/ml. It conveys low levels of resistance to kanamycin, at 1.5 ug/ml. E. coli NEB10B cells are sensitive to gentamicin at less than 0.3 ug/ml. Selection for this cassette carried out at 4 ug/ml will allow growth in Gentamicin, but inhibit other growth. With standard kanamycin concentrations of 35 ug/ml, this cassette will not convey Kanamycin resistance.
Gentamicin is an important antibiotic for use in human disease. Its use should be carefully considered and alternatives sought when used in a context promoting easy horizontal transfer of the cassette to potential human pathogens, for example in mobilizable plasmids or in transposable elements (insertions sequences).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]