Difference between revisions of "Part:BBa K343001:Design"
CKurtzhals (Talk | contribs) (→Design Notes) |
CKurtzhals (Talk | contribs) (→References) |
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Science 24 March 2000: 2222 | Science 24 March 2000: 2222 | ||
DOI: 10.1126/science.287.5461.2222 | DOI: 10.1126/science.287.5461.2222 | ||
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+ | [http://flybase.org/reports/FBcl0129473.html]FlyBase.org cDNA reference |
Latest revision as of 16:16, 2 July 2010
B-carotene monooxygenase, Produces retinal from B-carotene. ninaB gene from Drosophila.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 685
Illegal BamHI site found at 420
Illegal BamHI site found at 1677 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1281
Illegal BsaI site found at 1729
Design Notes
The sequence is isolated from a cDNA, starting from the first Met residue after the second to last stop codon of the correct reading frame, and ending at final stop. It matches the number of residues in the desired protein.
Source
Sequence taken from cDNA from Ruben GM, et. al.
References
[http://www.sciencemag.org/cgi/content/abstract/287/5461/2222A] Drosophila Complementary DNA Resource Rubin et al. Science 24 March 2000: 2222 DOI: 10.1126/science.287.5461.2222
[http://flybase.org/reports/FBcl0129473.html]FlyBase.org cDNA reference