Difference between revisions of "Part:BBa K249019:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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| + | When sequencing the stock we had from 2009 our team (Lethbridge) found a RBS mutation. We attempted to overexpress the protein and found that it would not. We do not know if this is the same for the kits that were sent out this year or not. We recommend that you sequence the part before use and if it has the same mutation do not use directly but mutate the mutation out. The mutation we found is as follows: | ||
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| + | Mms6 7 VF Prefix TCCCGACTGGAAAGCGGG-NNANAGNGCAACGCAATTAATGTGAGTTAGCTCACTCATTA | ||
| + | Mms6 7 VR A-Suffix (reversed) TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA | ||
| + | Mms6 8 VF Prefix TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA | ||
| + | Mms6 8 VR A-Suffix (reversed) TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA | ||
| + | mRBS TCAC-ACAGGAAAG---------------------------------------------- | ||
Revision as of 22:15, 28 June 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K249019
User Reviews
UNIQ987a44e245dd73ae-partinfo-00000000-QINU UNIQ987a44e245dd73ae-partinfo-00000001-QINU
When sequencing the stock we had from 2009 our team (Lethbridge) found a RBS mutation. We attempted to overexpress the protein and found that it would not. We do not know if this is the same for the kits that were sent out this year or not. We recommend that you sequence the part before use and if it has the same mutation do not use directly but mutate the mutation out. The mutation we found is as follows:
Mms6 7 VF Prefix TCCCGACTGGAAAGCGGG-NNANAGNGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 7 VR A-Suffix (reversed) TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 8 VF Prefix TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 8 VR A-Suffix (reversed) TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
mRBS TCAC-ACAGGAAAG----------------------------------------------