Difference between revisions of "Part:BBa J70595:Design"
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<partinfo>BBa_J70595 short</partinfo> | <partinfo>BBa_J70595 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Amplified via colony PCR directly from the BBa_E0040 cell stock. | + | Amplified via colony PCR directly from the BBa_E0040 cell stock. Note that both the ATG and double TAA codons were removed. |
Forward Primer: | Forward Primer: | ||
| − | 5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3' | + | 5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3', GFP-F |
Reverse Primer: | Reverse Primer: | ||
| − | + | 5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3', GFP-R | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | 5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3' | + | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 14:46, 23 June 2010
RFC12 Green Fluorescent Protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Design Notes
Amplified via colony PCR directly from the BBa_E0040 cell stock. Note that both the ATG and double TAA codons were removed.
Forward Primer:
5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3', GFP-F
Reverse Primer:
5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3', GFP-R
Source
BBa_E0040