Difference between revisions of "Part:BBa J70593:Design"
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<partinfo>BBa_J70593 short</partinfo> | <partinfo>BBa_J70593 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Made with synthetic | + | Made with synthetic oligos: |
| − | 5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F | + | 5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F |
| − | 5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R | + | 5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R |
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. | Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 18:28, 18 June 2010
RFC12 ATG Head Domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Made with synthetic oligos:
5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F
5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
Source
Common knowledge.