Difference between revisions of "Part:BBa J70593:Design"

 
(Design Notes)
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J70593 short</partinfo>
 
<partinfo>BBa_J70593 short</partinfo>
Line 9: Line 8:
 
Made with synthetic  
 
Made with synthetic  
  
5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F
+
5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F
  
5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R
+
5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R
  
 
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
 
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
 
 
 
 
  
 
===Source===
 
===Source===

Revision as of 18:28, 18 June 2010

RFC12 ATG Head Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with synthetic

5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F
5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Common knowledge.

References