Difference between revisions of "Part:BBa J70591:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J70591 short</partinfo> | <partinfo>BBa_J70591 short</partinfo> | ||
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Designed with synthetic oligos: | Designed with synthetic oligos: | ||
| − | + | 5' AATTC GCGGCGC T ACTAGT AAAGAGGAGAAA GCTAGC A GCGGCCG CTGCA 3', RBS-F | |
| − | 5' AATTC GCGGCGC T ACTAGT AAAGAGGAGAAA GCTAGC A GCGGCCG CTGCA 3' | + | |
| − | RBS- | + | |
| − | + | ||
| − | + | ||
| + | 5' GCGGCCGCTGCTAGC TTTCTCCTCTTT ACTAGTAGCGCCGCG 3', RBS-R | ||
| + | Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase. | ||
===Source=== | ===Source=== | ||
Latest revision as of 16:11, 18 June 2010
RFC12 Elowitz RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with synthetic oligos:
5' AATTC GCGGCGC T ACTAGT AAAGAGGAGAAA GCTAGC A GCGGCCG CTGCA 3', RBS-F
5' GCGGCCGCTGCTAGC TTTCTCCTCTTT ACTAGTAGCGCCGCG 3', RBS-R
Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.
Source
Part BBa_B0034