Difference between revisions of "Part:BBa K416002:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K416002 short</partinfo>
 
<partinfo>BBa_K416002 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The coding region of the gene was isolated via PCR. Biobrick prefix and suffix were added during amplification, and ligated into Ampicillin resistant plasmids.
+
This is a LoxP site, target of Cre Recombinase, normally 34-bp but here 2 bp were added so that the site can be translated in frame with a fusion protein.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 16:05, 16 June 2010

36 Base Pair LoxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a LoxP site, target of Cre Recombinase, normally 34-bp but here 2 bp were added so that the site can be translated in frame with a fusion protein.

Source

Amplified and biobricked from E. coli.

References