Difference between revisions of "Part:BBa J70500"

Latest revision as of 23:32, 29 May 2010

pWV01 broad host range plasmid origin and rep protein

pWV01 broad host range plasmid origin and rep protein. pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.

For use with mycoplasma species, the repA (orfD) seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan. This may need to be modified for use in the mycoplasma and mesoplasma species.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:31, 29 May 2010 (view source) Tk (Talk \| contribs) ← Older edit		Latest revision as of 23:32, 29 May 2010 (view source) Tk (Talk \| contribs)
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	pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.		pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.

−	For use with mycoplasma species, the orfD seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan. This may need to be modified for use in the mycoplasma and mesoplasma species.	+	For use with mycoplasma species, the repA (orfD) seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan. This may need to be modified for use in the mycoplasma and mesoplasma species.