Difference between revisions of "Part:BBa J70500"

 
 
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<partinfo>BBa_J70500 short</partinfo>
 
<partinfo>BBa_J70500 short</partinfo>
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pWV01 broad host range plasmid origin and rep protein.
 
pWV01 broad host range plasmid origin and rep protein.
 
pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species.  This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.
 
pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species.  This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.
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For use with mycoplasma species, the repA (orfD) seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan.  This may need to be modified for use in the mycoplasma and mesoplasma species.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:32, 29 May 2010

pWV01 broad host range plasmid origin and rep protein

pWV01 broad host range plasmid origin and rep protein. pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.

For use with mycoplasma species, the repA (orfD) seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan. This may need to be modified for use in the mycoplasma and mesoplasma species.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]