Difference between revisions of "Part:BBa J18932"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J18932 short</partinfo>
 
<partinfo>BBa_J18932 short</partinfo>
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* bright and photo-stable
 
* bright and photo-stable
  
 +
'''Degradation issues:'''
 +
 +
* Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
 +
* SDS treatment or boiling can hydrolyze the chromophore position at F//MYG splitting the protein in half
  
  

Revision as of 18:54, 26 January 2010

mCherry RFP

Red fluorescent protein derived from DsRed.

Advantages:

  • fast folding and maturation
  • bright and photo-stable

Degradation issues:

  • Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
  • SDS treatment or boiling can hydrolyze the chromophore position at F//MYG splitting the protein in half


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]