Difference between revisions of "Part:BBa J18911"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J18911 short</partinfo>
 
<partinfo>BBa_J18911 short</partinfo>
  
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Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 suffix but pointing outward into the backbone rather than insert.
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reverse primer for vector linearization of any vector containing the RFC 25 Biobrick flanks, anneals to prefix covering NgoMIV and XbaI site
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    * Tm (oligocalc nearest neighbore, 500nM primer, 50mM salt) = 60 C
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    * Tm (finnzyme, 500nM primer, 50mM salt) = 68
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    * Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 66.5
  
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Annealing temperature: 68 C (in Phusion polymerase buffer)
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See also: J18910 for forward
  
 
Example vectors:
 
Example vectors:

Revision as of 09:50, 25 January 2010

RFC 25 vector reverse (prefix)

reverse primer for vector linearization of any vector containing the RFC 25 Biobrick flanks, anneals to prefix covering NgoMIV and XbaI site 

   * Tm (oligocalc nearest neighbore, 500nM primer, 50mM salt) = 60 C

   * Tm (finnzyme, 500nM primer, 50mM salt) = 68

   * Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 66.5

See also: J18910 for forward

Example vectors: pSB1AC3F, pSB1AK3F, pSB1AT3F, pMA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9
    Illegal NgoMIV site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]