Difference between revisions of "Part:BBa K5443011"
 
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Feruloyl-CoA Synthetase activates ferulic acid by converting it into feruloyl-CoA as the third step in the vanillin biosynthesis pathway. The process involves the activation of FA through CoA-thioesterification. We tested two FCS enzymes for this pathway step; this enzyme from Pseudomonas, and also a homolog from Streptomyces.  
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<b>Feruloyl-CoA Synthetase</b> from <i>Pseudomonas putida</i>, <b>FCS-Ppu</b>, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.
  
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https://static.igem.wiki/teams/5443/fcs-reaction.jpg
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We tested two alternate FCS enzymes for this pathway step; this enzyme from <i>Pseudomonas</i>, and also a homolog from <i>Streptomyces</i> ([https://parts.igem.org/BBa_K5443012 BBa_K5443012]).
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This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: [https://parts.igem.org/Part:BBa_K1129002 BBa_K1129002]
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<html>
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<figure>
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    <img src="https://static.igem.wiki/teams/5443/fcs-reaction.jpg" style="width:600px;height:auto">
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    <figcaption>
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        <b>Figure 1. FCS-Ppu-catalysed conversion of ferulic acid to feruoyl-CoA.</b><br>
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        <i>This reaction represents the fourth step in our vanillin synthesis pathway. The enzyme FCS-Ppu converts ferulic acid into feruoyl-CoA.</i>
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    </figcaption>
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</figure>
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</html>

Latest revision as of 13:19, 2 October 2024

Feruloyl-CoA Synthetase from Pseudomonas putida, FCS-Ppu, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.

We tested two alternate FCS enzymes for this pathway step; this enzyme from Pseudomonas, and also a homolog from Streptomyces (BBa_K5443012).

This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: BBa_K1129002

Figure 1. FCS-Ppu-catalysed conversion of ferulic acid to feruoyl-CoA.
This reaction represents the fourth step in our vanillin synthesis pathway. The enzyme FCS-Ppu converts ferulic acid into feruoyl-CoA.