Difference between revisions of "Part:BBa K5443011"
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+ | <b>Feruloyl-CoA Synthetase</b> from <i>Pseudomonas putida</i>, <b>FCS-Ppu</b>, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification. | ||
− | + | We tested two alternate FCS enzymes for this pathway step; this enzyme from <i>Pseudomonas</i>, and also a homolog from <i>Streptomyces</i> ([https://parts.igem.org/BBa_K5443012 BBa_K5443012]). | |
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− | + | This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: [https://parts.igem.org/Part:BBa_K1129002 BBa_K1129002] | |
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− | == | + | <figure> |
− | + | <img src="https://static.igem.wiki/teams/5443/fcs-reaction.jpg" style="width:600px;height:auto"> | |
− | < | + | <figcaption> |
− | < | + | <b>Figure 1. FCS-Ppu-catalysed conversion of ferulic acid to feruoyl-CoA.</b><br> |
− | < | + | <i>This reaction represents the fourth step in our vanillin synthesis pathway. The enzyme FCS-Ppu converts ferulic acid into feruoyl-CoA.</i> |
− | + | </figcaption> | |
− | + | </figure> | |
− | < | + | </html> |
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Latest revision as of 13:19, 2 October 2024
Feruloyl-CoA Synthetase from Pseudomonas putida, FCS-Ppu, catalyses the fourth step in our vanillin biosynthesis pathway. It converts ferulic acid into feruoyl-CoA, avtivating it through CoA-thioesterification.
We tested two alternate FCS enzymes for this pathway step; this enzyme from Pseudomonas, and also a homolog from Streptomyces (BBa_K5443012).
This part builds on the work of earlier iGEM teams, who contributed FCS Parts as follows: BBa_K1129002