Difference between revisions of "Part:BBa K5443014:Experience"
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
We showed that the ECH-Str enzyme was functional in E.coli as part of a vanillin biosynthetic pathway, as evidenced by production of vanillin (see LC-MS data from one clone pMQ3C-11).  
+
===Applications of BBa_K5443014===
 +
We showed that the ECH-Str enzyme from <i>Streptomyces</i> was functional in <i>E. coli</i> as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).
  
−
https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg
+
As functional ECH-Str is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.
 +
 
 +
<html>
 +
<figure>
 +
    <img src="https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg" style="width:600px;height:auto">
 +
    <figcaption>
 +
        <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br>
 +
        <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Str.</i>
 +
    </figcaption>
 +
</figure>
 +
</html>

Latest revision as of 13:16, 2 October 2024

Applications of BBa_K5443014

We showed that the ECH-Str enzyme from Streptomyces was functional in E. coli as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).

As functional ECH-Str is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.

Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Str.