Difference between revisions of "Part:BBa K5322025:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence. | + | To ensure the successful expression of SOD-1 in <i>Escherichia coli</i>, we performed codon optimization of the SOD-1 sequence. |
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===Source=== | ===Source=== | ||
+ | <html> | ||
− | + | We obtained the SOD-1 sequence from <a href="https://parts.igem.org/Part:BBa_K2215003">BBa_K2215003</a>. | |
− | + | </html> | |
===References=== | ===References=== | ||
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1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA. | 1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA. | ||
− | 2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(),49-60.https://doi.org/10.1016/j.ymben.2022.09.001 | + | 2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(2),49-60.https://doi.org/10.1016/j.ymben.2022.09.001 |
3.4.Ge Qu, Jing Zhao, elt. Recent advances in directed evolution. Chinese Journal of Biotechnology. Jan. 25, 2018, 34(1): 1−11 | 3.4.Ge Qu, Jing Zhao, elt. Recent advances in directed evolution. Chinese Journal of Biotechnology. Jan. 25, 2018, 34(1): 1−11 |
Latest revision as of 13:10, 2 October 2024
SOD Plus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 412
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
Source
We obtained the SOD-1 sequence from BBa_K2215003.
References
1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA.
2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(2),49-60.https://doi.org/10.1016/j.ymben.2022.09.001
3.4.Ge Qu, Jing Zhao, elt. Recent advances in directed evolution. Chinese Journal of Biotechnology. Jan. 25, 2018, 34(1): 1−11