Difference between revisions of "Part:BBa K5322025:Design"
(→‎Source)
 
(4 intermediate revisions by 2 users not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
+
To ensure the successful expression of SOD-1 in <i>Escherichia coli</i>, we performed codon optimization of the SOD-1 sequence.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
 +
<html>
  
−
 
+
We obtained the SOD-1 sequence from <a href="https://parts.igem.org/Part:BBa_K2215003">BBa_K2215003</a>.
−
The team obtains the SOD-1 sequence from BBa_K2215003.
+
</html>
  
 
===References===
 
===References===
Line 21: Line 22:
 
1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA.  
 
1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA.  
  
−
2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(),49-60.https://doi. org/10.1016/j.ymben.2022.09.001
+
2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(2),49-60.https://doi.org/10.1016/j.ymben.2022.09.001
 +
 
 +
3.4.Ge Qu, Jing Zhao, elt. Recent advances in directed evolution. Chinese Journal of Biotechnology. Jan. 25, 2018, 34(1): 1−11

Latest revision as of 13:10, 2 October 2024


SOD Plus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.


Source

We obtained the SOD-1 sequence from BBa_K2215003.

References

1.Combinatorial alanine-scanning Kim L Morrison and Gregory A Weiss.Department of Chemistry, University of California, Irvine,CA 92697-2025, USA.

2.Kim,D. , Noh,M.H. , Park,M. , Kim,I. , Ahn,H. , Ye,D. , Jung,G.Y. ,& Kim , S.(2022).Enzyme activity engineering based on sequence co-evolution analysis.Metabolic Engineering,74(2),49-60.https://doi.org/10.1016/j.ymben.2022.09.001

3.4.Ge Qu, Jing Zhao, elt. Recent advances in directed evolution. Chinese Journal of Biotechnology. Jan. 25, 2018, 34(1): 1−11