Difference between revisions of "Part:BBa K5443013:Experience"
Nickcoleman (Talk | contribs) |
m |
||
(3 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
− | We showed that the ECH-Ppu | + | ===Applications of BBa_K5443013=== |
+ | We showed that the ECH-Ppu enzyme from <i>Pseudomonas putida</i> was functional in <i>E. coli</i> as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1). | ||
+ | |||
+ | As functional ECH-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11. | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <figure> | ||
+ | <img src="https://static.igem.wiki/teams/5443/pmq3c11-lcms-data-v2.jpg" style="width:600px;height:auto"> | ||
+ | <figcaption> | ||
+ | <b>Figure 1. LCMS analysis of compounds produced using pMQ3C-11.</b><br> | ||
+ | <i>The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Ppu.</i> | ||
+ | </figcaption> | ||
+ | </figure> | ||
+ | </html> |
Latest revision as of 13:08, 2 October 2024
Applications of BBa_K5443013
We showed that the ECH-Ppu enzyme from Pseudomonas putida was functional in E. coli as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).
As functional ECH-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.