Difference between revisions of "Part:BBa K5233022:Experience"

(→‎Applications of BBa_K5233022)
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===Applications of BBa_K5233022===
 
===Applications of BBa_K5233022===
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In our application, we design to express this basic part in E. coli, and our designed expression pathway is shown as follow :
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https://static.igem.wiki/teams/5233/design/dshc-3.png
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This is the plasmid map of turboPETase(OmpA):
  
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This is the plasmid map of the secretion of TurboPETase, containing our basic part turboPETase(ompA):
 
 
https://static.igem.wiki/teams/5233/parts/turbopetase-ompa-2.png
 
https://static.igem.wiki/teams/5233/parts/turbopetase-ompa-2.png
  
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After the construction of the plasmid, we successfully express the engineering E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in :
+
After the construction of the plasmid, we successfully express the engineered E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in :
 
https://2024.igem.wiki/hust-ueve-upsaclay/protocol
 
https://2024.igem.wiki/hust-ueve-upsaclay/protocol
  

Latest revision as of 13:04, 2 October 2024


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K5233022

In our application, we design to express this basic part in E. coli, and our designed expression pathway is shown as follow : dshc-3.png


This is the plasmid map of turboPETase(OmpA):

turbopetase-ompa-2.png

After the construction of the plasmid, we successfully express the engineered E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : https://2024.igem.wiki/hust-ueve-upsaclay/protocol

The results of the enzyme activity is shown as follows:

hplc2.png

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