Difference between revisions of "Part:BBa K5233022:Experience"
Lambertbai (Talk | contribs) |
Lambertbai (Talk | contribs) (→Applications of BBa_K5233022) |
||
| (7 intermediate revisions by the same user not shown) | |||
| Line 5: | Line 5: | ||
===Applications of BBa_K5233022=== | ===Applications of BBa_K5233022=== | ||
| + | In our application, we design to express this basic part in E. coli, and our designed expression pathway is shown as follow : | ||
| + | https://static.igem.wiki/teams/5233/design/dshc-3.png | ||
| + | |||
| + | |||
| + | This is the plasmid map of turboPETase(OmpA): | ||
| + | |||
| + | https://static.igem.wiki/teams/5233/parts/turbopetase-ompa-2.png | ||
| + | |||
| + | After the construction of the plasmid, we successfully express the engineered E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : | ||
| + | https://2024.igem.wiki/hust-ueve-upsaclay/protocol | ||
| + | |||
| + | The results of the enzyme activity is shown as follows: | ||
| + | |||
| + | https://static.igem.wiki/teams/5233/parts/hplc2.png | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K5233022 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K5233022 StartReviews</partinfo> | ||
<!-- Template for a user review | <!-- Template for a user review | ||
| − | {|width=' | + | {|width='70%' style='border:1px solid gray' |
| − | + | ||
| − | + | ||
|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K5233022 AddReview number</partinfo> | <partinfo>BBa_K5233022 AddReview number</partinfo> | ||
Latest revision as of 13:04, 2 October 2024
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K5233022
In our application, we design to express this basic part in E. coli, and our designed expression pathway is shown as follow :
This is the plasmid map of turboPETase(OmpA):
After the construction of the plasmid, we successfully express the engineered E.coli that can secrete the turboPETase.After that, we test the enzyme activity and compare the enzyme activity with other TurboPETases which have different siganl peptide, the process is done by mixing the bacteria with BHET substrate solution,and the detection is done by HPLC. the details procedures are shown in : https://2024.igem.wiki/hust-ueve-upsaclay/protocol
The results of the enzyme activity is shown as follows:
User Reviews
UNIQ437bce71474a6cb1-partinfo-00000000-QINU UNIQ437bce71474a6cb1-partinfo-00000001-QINU