Difference between revisions of "Part:BBa K5291022"

Latest revision as of 12:59, 2 October 2024

PcW

A common weak constitutive promoter in Pseudomonas aeruginosa.

Usage and Biology

We select PcW as the replacing promoter of hok/mok through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of sok is stronger than hok/mok when there is citrate in the environment, preventing cell death in advanced.

Fig.1 The schema of hok/sok system. The promoter of hok/mok is PcW.

We produce the hok/sok system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-We select PcW as the replacing promoter of <i>hok</i>/<i>mok</i> through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of <i>sok</i> is stronger than <i> hok</i>/<i>mok</i> when there is citrate in the environment, preventing cell death in advanced.
+We select PcW as the replacing promoter of <i>hok</i>/<i>mok</i> through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of <i>sok</i> is stronger than <i> hok</i>/<i>mok</i> when there is citrate in the environment, preventing cell death in advanced.<br><br>
+<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html>
+<br><b>Fig.1 The schema of <i>hok</i>/<i>sok</i> system. The promoter of <i>hok</i>/<i>mok</i> is PcW.</b><br><br>
+We produce the <i>hok</i>/<i>sok</i> system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037.
 <br><br>