Difference between revisions of "Part:BBa K5291022"

 
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<partinfo>BBa_K5291022 short</partinfo>
 
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PcW is a common promoter discovered in Pseudomonas aeruginosa, whose characteristic is a low degree of gene activating ability, ensuring a decent expression of hok/sok. As a constitutive promoter, PcW is able to come into effect at any situations, which secures the normal expression of hok mRNA when the concentration of citrate is down and sok stops expressing.
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A common weak constitutive promoter in <i>Pseudomonas aeruginosa</i>.
  
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===Usage and Biology===
 
===Usage and Biology===
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We select PcW as the replacing promoter of <i>hok</i>/<i>mok</i> through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of <i>sok</i> is stronger than <i> hok</i>/<i>mok</i> when there is citrate in the environment, preventing cell death in advanced.<br><br>
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<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html>
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<br><b>Fig.1 The schema of <i>hok</i>/<i>sok</i> system. The promoter of <i>hok</i>/<i>mok</i> is PcW.</b><br><br>
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We produce the <i>hok</i>/<i>sok</i> system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037.
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Latest revision as of 12:59, 2 October 2024


PcW

A common weak constitutive promoter in Pseudomonas aeruginosa.

Usage and Biology

We select PcW as the replacing promoter of hok/mok through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of sok is stronger than hok/mok when there is citrate in the environment, preventing cell death in advanced.


Fig.1 The schema of hok/sok system. The promoter of hok/mok is PcW.

We produce the hok/sok system as a whole therefore there is no figures of PcW alone. You are able to know about the results relating to PcW in the part BBa_K5291037.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]