Difference between revisions of "Part:BBa K5291011"

 
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<partinfo>BBa_K5291011 short</partinfo>
 
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This gene comes from the hok/sok system in Escherichia coli. It is able to encode a type of antisense RNA that can specifically bind to the hok mRNA, repressing its translation. Therefore, the toxic protein cannot be produced in normal conditions, and the engineered bacteria will survive. It's worth paying attention is that the half-life of sok RNA is much shorter than hok mRNA, which lead to the situation that when both of them cannot express, sok RNA is completely degraded while mok mRNA still exist, making the toxic protein be encoded and bacteria be killed. We take advantage of this characteristic to design a suicide system.
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The gene encoding a type of antisense RNA that can bind to the <i>hok</i> mRNA and repress the production of toxic potein.
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===Usage and Biology===
 
===Usage and Biology===
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The gene <i>sok</i> from <i>hok</i>/<i>sok</i> system can encode a kind of antisense RNA that can bind to <i>hok</i> mRNA to repress the translation of toxic protein.<br><br>
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<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html><br>
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<b>Fig.1 The schema of <i>sok</i> (the gene on the left)</b><br><br>
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<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-normal.png" /></html><br><br>
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<b>Fig.2 The mechanism of <i>hok</i>/<i>sok</i> system</b><br>
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<html><img width = "300" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-pcr-arm2.png" /></html><br>
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<b>Fig.3 The AGE figure of <i>hok</i>/<i>sok</i></b><br><br>
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For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of the whole system.<br><br>
  
 
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Latest revision as of 12:56, 2 October 2024


sok

The gene encoding a type of antisense RNA that can bind to the hok mRNA and repress the production of toxic potein.


Usage and Biology

The gene sok from hok/sok system can encode a kind of antisense RNA that can bind to hok mRNA to repress the translation of toxic protein.


Fig.1 The schema of sok (the gene on the left)



Fig.2 The mechanism of hok/sok system

Fig.3 The AGE figure of hok/sok

For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of the whole system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]