Difference between revisions of "Part:BBa K5291010"

 
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<partinfo>BBa_K5291010 short</partinfo>
 
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This gene comes from the hok/sok system in Escherichia coli. The hok is able to encode a kind of toxic protein that can result to the collapse of cell membrane potential, cellular respiratory arrest, cell shape changes, and ultimately cell death. The gene mok is overlapped with hok, and it encodes the lead peptide of the toxic protein. Both of them have a relatively longer half-life. The hok mRNA can be specifically binded with sok aitisense RNA, which inhibits the expression of the toxic protein.
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The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by <i>sok</i> antisense RNA.
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===Usage and Biology===
 
===Usage and Biology===
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The gene <i>hok</i>/<i>mok</i> from <i>hok</i>/<i>sok</i> system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And <i>sok</i>, the gene also in <i>hok</i>/<i>sok</i> system, can encode a kind of antisense RNA to specifically bind to <i>hok</i> mRNA, repressing the expression of toxin.<br><br>
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<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksoknew.png" /></html><br>
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<b>Fig.1 The schema of <i>hok</i>/<i>mok</i> (the gene on the right)</b><br><br>
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<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-normal.png" /></html><br><br>
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<b>Fig.2 The mechanism of <i>hok</i>/<i>sok</i> system</b><br>
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<html><img width = "300" src="https://static.igem.wiki/teams/5291/images/part-cr/hoksok-pcr-arm2.png" /></html><br>
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<b>Fig.3 The AGE figure of <i>hok</i>/<i>sok</i></b><br><br>
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For convenience, we set <i>hok</i>/<i>mok</i> and <i>sok</i> as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of <i>hok</i>/<i>mok</i>.<br><br>
  
 
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Latest revision as of 12:43, 2 October 2024


hok/mok

The gene encoding a kind of toxic protein that can lead to bacteria death, whose mRNA will be repressed by sok antisense RNA.


Usage and Biology

The gene hok/mok from hok/sok system can encode a kind of toxic protein that can lead to cell death. We take advantage of this characteristic and take it as the switch of the suicide system. And sok, the gene also in hok/sok system, can encode a kind of antisense RNA to specifically bind to hok mRNA, repressing the expression of toxin.


Fig.1 The schema of hok/mok (the gene on the right)



Fig.2 The mechanism of hok/sok system

Fig.3 The AGE figure of hok/sok

For convenience, we set hok/mok and sok as a whole and didn't prepare both of them separately. You can refer to the part BBa_K5291037 to get to know about the function of hok/mok.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]