Difference between revisions of "Part:BBa K5291006"
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<partinfo>BBa_K5291006 short</partinfo>
 
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The gene acaP comes from Rhodopseudomonas palustris CGA009, and it can encode a kind of &#945;-carbonic anhydrase that is able to speed up the reversible conversion between CO2 and bicarbonate. In our design, engineered Pseudomonas aeruginosa PAO1 converse CO2 into bicarbonate, in order to deliver the carbon source in the biofilm more easily. Rhodopseudomonas palustris CGA009 take in the bicarbonate and transform it as CO2 for CBB cycle. The efficiency of the conversion in Rhodopseudomonas palustris WT is limited. Therefore, we introduce acaP into Rhodopseudomonas palustris CGA009 to realize overexpressing of the carbonic anhydrase, which enable the engineered Rhodopseudomonas palustris to converse bicarbonate into CO2 in a higher rate, accelerating the process of carbon fixation in Rhodopseudomonas palustris CGA009.
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A gene from <i>Rhodopseudomonas palustris</i> CGA009 encoding a kind of carbonic anhydrase.
  
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===Usage and Biology===
 
===Usage and Biology===
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The gene <i>acaP</i> in <i>Rhodopseudomonas palustris</i> is able to encode a kind of α-CAs that can speed up the conversion between CO<sub>2</sub> and bicarbonate. By the method of <i>acaP</i> high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO<sub>2</sub> conversion in <i>R. palustris</i> increase and the engineered bacteria is able to take advantage of carbon to produce cellulose.<br><br>
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<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/acap-schema.png" /></html><br>
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<b>Fig.1 The schema of <i>acaP</i>.</b><br><br>
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<html><img width = "700" src="https://static.igem.wiki/teams/5291/images/part-cr/pbbr1mcs2-rca.png" /></html><br>
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<b>Fig.2 The map of pBBR1MCS2-<i>acaP</i>.</b><br><br>
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However, there is a great pity that we have not finished the verification of the function due to rush time.<br><br>
  
 
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Latest revision as of 12:34, 2 October 2024


acaP

A gene from Rhodopseudomonas palustris CGA009 encoding a kind of carbonic anhydrase.

Usage and Biology

The gene acaP in Rhodopseudomonas palustris is able to encode a kind of α-CAs that can speed up the conversion between CO2 and bicarbonate. By the method of acaP high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO2 conversion in R. palustris increase and the engineered bacteria is able to take advantage of carbon to produce cellulose.


Fig.1 The schema of acaP.


Fig.2 The map of pBBR1MCS2-acaP.

However, there is a great pity that we have not finished the verification of the function due to rush time.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 223
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 223
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 223
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 223
  • 1000
    COMPATIBLE WITH RFC[1000]